TABLE 1.

Primers and thermal cycle parameters for cloning of M. tuberculosis antigens

Antigen	Primer orientation or sequence (5′-3′)	PCR parameters	Amplicon size (bp)
CFP21F	AAGGATCCGATCCGTGTTCGGACATCGCGGTCG	94°C for 5 min, then 30 cycles at 94°C for	608
CFP21R	GCTGCCGCCACCGCCGCTTCCGCCACCGCCGCTTCCACCGCCACCTCCGGCGTGATCGAGCCTGTTCGCC	1 min, 60°C for 1 min, and 72°C for 45 s, then 72°C for 5 min
MPT64F	GGTGGCGGTGGAAGCGGCGGTGGCGGAAGCGGCGGTGGCGGCAGCGCGCCCAAGACCTACTGCGAGGAG	94°C for 5 min, then 30 cycles at 94°C for 1 min, 60°C for 1 min, and 72°C for	671
MPT64R	GAAAGCTTCTAGGCCAGCATCGAGTCGATCGC	45 s, then 72°C for 5 min
rCMF	CFP21F	94°C for 5 min, then 30 cycles at 94°C for	1,234
rCMR	MPT64R	1 min, 60°C for 1 min, and 72°C for 90 s, then 72°C for 10 min