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. 1999 Mar 2;96(5):2514–2519. doi: 10.1073/pnas.96.5.2514

Figure 3.

Figure 3

Figure 3

In situ hybridization of sgk in rat kidney. Adx rats were treated for 4 h with vehicle (Adx, n = 5) or aldosterone (Adx + Aldo, n = 5). Longitudinal frozen sections (15 μm) were cut and hybridized with a rat sgk RNA probe (see Materials and Methods). (A) Autoradiagram of whole kidney. (B–F) Emulsion-dipped sections counterstained with hematoxylin and eosin at ×200 magnification (except D, which is ×400). A darkfield view is shown immediately beneath each light micrograph. Sense controls performed on sections from the adx group are shown for each kidney region. Sense controls for adx + aldo showed similar levels of signal (not shown). (B) Glomerulus (G). (C) Cortical tubules [proximal tubule (PT); distal tubule/collecting duct (DT)]. (D) ×400 magnification of cortex showing a tubule with morphology consistent with distal nephron. (E) Outer medulla. (F) Inner medulla/papilla.