Skip to main content
. 2009 Mar 13;8(5):703–712. doi: 10.1128/EC.00333-08

FIG. 6.

FIG. 6.

Response of cytoplasmic pH to a sudden extracellular pH change. Cytoplasmic pH measurement and fluorescence ratio imaging of cytoplasmic pH in growing hyphae expressing RaVC from 18-h-old cultures. (A) Effects of a strong acid (50 mM HCl) or base (5 mM KOH) on cytoplasmic pH. (C) Effects of sorbic acid (5 or 10 mM) on cytoplasmic pH. (B and D) Pseudocolored fluorescence ratio images of hyphae. The pseudocolors correspond to defined pH values calculated from the fluorescence ratios using the in situ calibration (blue, alkaline; red, acidic). Bars = 10 μm.