FIG. 3.

Oxidant sensitivity of skn7 mutants. Yeast strain JF1904 (skn7Δ) was transformed with low-copy (A) or high-copy (B) expression plasmids carrying wild-type or mutated SKN7 genes fused to sequences encoding the Myc epitope tag. Tenfold serial dilutions starting at 10⁶ cells/ml were spotted, and the plates were incubated at 30°C for 2 to 3 days. (C and D) α-myc Western analysis showing normal protein levels in the skn7 mutant strains. Ponceau S staining (C) or anti-Hog1 Western analysis (D) was used to confirm the equal loading of samples. Low-copy plasmids were the following: SKN7⁺, pXH1853; skn7Δ, pRS315; skn7-T437A, pXH1854; skn7-T455A, pXH1855; skn7-T449A, pXH1856; and skn7-D427N, pXH1858. High-copy plasmids were the following: SKN7⁺, pXH1938; skn7Δ, pRS425; and skn7-T449A, pXH1939.