FIG. 4.
skn7 receiver domain (RD) mutations affect Skn7 transcriptional activation in response to oxidant. Fusions of the Skn7 C-terminal sequence (aa 351 to 622; wild-type [WT] and mutant derivatives as indicated) to the DBD of Gal4 were introduced into wild-type yeast strain PJ69-4A (12), which carries the UASG-lacZ reporter. The Gal4 DBD plasmid (pGBD-C2) also was introduced as a negative control. The yap1Δ strain was generated from PJ69-4A (yap1Δ::KAN). Log-phase cultures were untreated or were treated with 0.5 mM t-BOOH for 30 min (white bars, untreated; filled bars, treated). β- Galactosidase activities are given in Miller units (23) normalized to the protein concentration and are the average activities of three or four independent transformants. Error bars are the standard deviations from the means. Below the plot is the induction (n-fold) due to oxidant treatment.