FIG. 5.

Effect of skn7 mutations on the SLN1-SKN7 target genes. Total RNA (15 μg) was isolated from the log-phase cultures of the skn7Δ yeast strains (SLN1⁺ [JF1904] and sln1* [JF2052]) carrying the indicated SKN7 expression plasmids or empty vector (skn7Δ) and subjected to formaldehyde agarose gel electrophoresis. The expression of the OCH1 and NCA3 genes was determined by hybridization to the appropriate probes and to a PGK1 probe for normalization. Transcript levels were determined using Quantity One software (Bio-Rad). The expression of the untreated SKN7⁺ culture was set to 1.0. Induction values (averages from at least three experiments) are plotted and provided below the x axis for each pair of bars. Asterisks indicate induction values that differ significantly (P < 0.05) from that of SKN7⁺. Plasmids carrying SKN7 alleles were the following: SKN7⁺, pXH1853; skn7-T437A, pXH1854; skn7-T449A, pXH1856; and skn7-D427N, pXH1858.