FIG. 6.

Effect of receiver domain mutations on Skn7 DNA binding. Shown are electrophoretic mobility shift assays in which protein extracts were prepared from YAP1 skn7Δ (JF1904) or yap1Δ skn7Δ (JF2312) yeast strains carrying wild-type or mutant derivatives of SKN7 and incubated with a labeled DNA probe corresponding to positions −355 to −154 of the OCH1 promoter. This fragment of the OCH1 promoter contains Skn7 but no Yap1 binding sites. Complexes were separated by nondenaturing polyacrylamide gel electrophoresis at 200 V for 2 h. A single SKN7-dependent complex is labeled with an arrow. Plasmids carrying SKN7 alleles were the following: SKN7⁺, pXH1853; skn7-T437A, pXH1854; skn7-T449A, pXH1856; skn7-T437S, pXH1857; and skn7-D427N, pXH1858.