FIG. 8.

Dock mutant is oxidative stress sensitive and defective in Skn7-Yap1 complex formation. (A) Tenfold serial dilutions starting at 10⁶ cells/ml were spotted on media with and without oxidant, and the plates incubated at 30°C for 2 to 3 days. The skn7Δ strain, JF1904, was transformed with the following high-copy SKN7 expression plasmids: SKN7⁺, pXH1938; skn7Δ, pRS425; and skn7-dock2, pXH1941. (B) α-myc Western analysis showing normal protein levels in the skn7-dock2 mutant. (C) Gel shift analysis of the skn7-dock2 mutant with CCP1 promoter sequences containing both Skn7 and Yap1 binding sites. S/Y, complex dependent on both Skn7 and Yap1; S, complex dependent on Skn7; Y, complex dependent on Yap1. The S and Y complexes from YAP1⁺ extracts comigrate.