TABLE 1.
Plasmids used in this study
| Plasmid | Descriptiona | Vector | Reference |
|---|---|---|---|
| PXH1641 | CCP1 (−309/+14)-lacZ | pSEYC102 | 11 |
| PXH1801 | SKN7 (WT) | pRS315 | 32 |
| PXH1833 | skn7-C160A | pRS315 | |
| PXH1834 | skn7-C300A | pRS315 | |
| PXH1835 | skn7-C392A | pRS315 | |
| PXH1836 | skn7-C401A | pRS315 | |
| PXH1837 | skn7-C499A | pRS315 | |
| PXH1853 | SKN7 (WT) (myc2x)b | pRS315 | |
| PXH1854 | skn7-T437A (myc2x) | pRS315 | |
| PXH1855 | skn7-T455A (myc2x) | pRS315 | |
| PXH1856 | skn7-T449A (myc2x) | pRS315 | |
| PXH1857 | skn7-T437S (myc2x) | pRS315 | |
| PXH1858 | skn7-D427N (myc2x) | pRS315 | |
| PXH1946 | skn7-T437E (myc2x) | pRS315 | |
| PXH1859 | SKN7 (WT) (GFP) | pRS315 | |
| PXH1860 | skn7-T437A (GFP) | pRS315 | |
| PXH1861 | skn7-T455A (GFP) | pRS315 | |
| PXH1862 | skn7-T449A (GFP) | pRS315 | |
| PXH1863 | skn7-T437S (GFP) | pRS315 | |
| PXH1864 | skn7-D427N (GFP) | pRS315 | |
| PXH1938 | SKN7 (WT) (myc2x) (high copy) | pRS425 | |
| PXH1939 | skn7-T449A (myc2x) (high copy) | pRS425 | |
| PXH1941 | skn7-dock2 (myc2x) (high copy) | pRS425 | |
| PXH1956 | GAL4 DBD-SKN7 (+1051/+1866) (WT) (myc2x) | pBD-C(2) | 12 |
| PXH1966 | GAL4 DBD-skn7 (+1051/+1866)-T437A (myc2x) | pBD-C(2) | |
| PXH1967 | GAL4 DBD-skn7 (+1051/+1866)-T455A (myc2x) | pBD-C(2) | |
| PXH1968 | GAL4 DBD-skn7 (+1051/+1866)-T449A (myc2x) | pBD-C(2) | |
| PXH1970 | GAL4 DBD-skn7 (+1051/+1866)-D427N (myc2x) | pBD-C(2) | |
| pSEY18-YAP1 | YAP1 (high copy) | pSEY18 |
a
WT, wild type.
b
myc2x, two copies of the myc epitope tag.