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Aberrant plasma membrane accumulation of Hxt2 in rvs161 cells can be suppressed by the loss of SUR4. Cells were grown to exponential phase at 30°C in YEPD and then sequentially shifted to medium containing the indicated sugar concentrations for the indicated times. Hxt2-GFP localization was visualized by live-cell fluorescence microscopy. WT, wild type; Glu, glucose; hr, hours.
