FIG. 5.

Sugar transporter endocytosis defects of rvs161 cells can be suppressed by the loss of SUR4. Cells were grown to exponential phase at 30°C in YEPD (A and B) or YEP containing 2% galactose (C). They then were sequentially shifted to medium containing the indicated sugar concentrations for the times indicated. Cell lysates were resolved by SDS-PAGE, and sugar transporter levels were determined by Western analysis using anti-GFP polyclonal antibodies (A and B) or anti-Gal2 polyclonal antibodies (C). Act1 (actin) levels were used as a loading control. WT, wild type; Glu, glucose; hr, hours. (A) Hxt2-GFP; (B) Hxt1-GFP; (C) Gal2.