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rvs161 cells hyperaccumulate monoubiquitin, and the loss of SUR4 suppresses this defect. Whole-cell lysates were resolved by SDS-PAGE, and monoubiquitin and high-molecular-weight polyubiquitin conjugates were visualized immunologically using anti-ubiquitin polyclonal antibodies. The levels of various ubiquitin species were normalized against actin using densitometry analysis. The values are the averages from three experiments ± standard deviations.
