TABLE 1.
The effects of genetic knockdown of polyamine biosynthetic enzymes on polyamine levels and the biosynthetic enzymes in blood form T. brucei
| Genetic knockdown/chemical inhibitiona | Cell response (% of the wild-type level)b
|
||||||
|---|---|---|---|---|---|---|---|
| Putrescine | Spermidine | GSH-Spd | T(SH)2 | AdoMet | dcAdoMet | Pathway enzymes | |
| ODC | 0 | 50 | <5 | <5 | 100 | 400 | nc |
| DFMO treatment | 0 | 40 | 20 | 40 | 100 | 4,000 | nc |
| SpdSyn | 30 | 20 | <5 | <5 | 100 | 18,000 | nc |
| AdoMetDCc | 700 | 40 | <5 | <5 | ND | ND | 2,500, prozyme; 700, ODC |
| Prozymec | 1,000 | <5 | <5 | <5 | ND | ND | 500, ODC |
a
For ODC, SpdSyn, and AdoMetDC, the results are from RNAi-mediated gene silencing, while for prozyme, the results are taken from a gene knockout cell line expressing a Tet-inducible regulated copy of the gene. For ODC, data represent the 48-h time point after Tet induction; for SpdSyn and AdoMetDC, data represent the 6-day time point after Tet induction; and for prozyme, data represent the 4-day time point after Tet withdrawal.
b
ND, not determined; nc, no significant change.
c
Data taken from reference 40.