FIG. 1.

Approximate location of lineage II-specific regions with respect to the E. coli O157:H7 strain Sakai genome. Contigs containing lineage II-specific regions together with regions with identity to the Sakai genome were used to place the lineage II-specific regions on the circular map, using the CGView program (43). Red regions represent lineage II-specific loci which are centered at their approximate insertion points with respect to the Sakai genome. Twenty-five of the 27 primers were placed on the Sakai genome in an analogous fashion. Two primer sets (B01 and B18) could not be placed on the map because they targeted lineage II-specific contigs that were missing sequences with identity to the Sakai genome to serve as reference points.