TABLE 3.
Frequency of sequence classes within SSH DNA libraries
| Sequence classa | No. (%) of clones present in:
|
Range | Avg no. of clones/contig | |||
|---|---|---|---|---|---|---|
| All contigs (n = 811) | 3 SSH (n = 16) | 2 SSH (n = 73) | 1 SSH (n = 722) | |||
| A | 503 (62.0) | 5 (31.3) | 30 (41.1) | 468 (64.7) | 1-7 | 1.30 ± 0.82 |
| B | 92 (11.3) | 10 (66.7) | 34 (46.6) | 48 (6.6) | 1-16 | 2.90 ± 2.64 |
| C | 72 (8.9) | 3 (18.8) | 12 (16.4) | 57 (7.9) | 1-7 | 1.60 ± 1.22 |
| D | 19 (2.3) | 0 (0) | 6 (8.2) | 13 (1.8) | 1-5 | 1.79 ± 1.13 |
| E | 80 (9.9) | 1 (13.3) | 8 (12.7) | 72 (9.7) | 1-27 | 3.01 ± 4.67 |
| F | 188 (23.3) | 5 (31.3) | 14 (19.2) | 177 (24.5) | 1-11 | 1.67 ± 1.65 |
| All | 1-27 | 1.58 ± 1.90 | ||||
a
Class A, sequences with homology to E. coli Sakai (14) genes; class B, sequences with homology to bacteriophage sequences not found in E. coli Sakai; class C, putative mosaic sequences with homology to both E. coli Sakai and non-Sakai DNA sequences within the same contig; class D, sequences with homology to E. coli Sakai genes but lacking synteny with the E. coli Sakai chromosome; class E, sequences with homology to previously published nonbacteriophage and non-Sakai DNA sequences; class F, sequences with regions containing no homology to any annotated DNA sequences in GenBank.