TABLE 2.
All-Bacteria and Bacteroidales markers tested with different fecal and effluent samples from human, pig, and other animal sources
| Source of sample (no. of samples) | % of samples positivea with indicated probe
|
|||||
|---|---|---|---|---|---|---|
| All Bacteria; Bact2 | All Bacteroidales; AllBac | Pig-specific Bacteroidales
|
Human-specific Bacteroidales; HF183 | Ruminant-specific Bacteroidales; BacR | ||
| Pig-1-Bac | Pig-2-Bac | |||||
| Pig | ||||||
| Feces (25) | 100 | 100 | 100 | 100 | 0 | 0 |
| Slurry (23) | 100 | 100 | 100 | 100 | 0 | 17 |
| Lagoon water (14) | 100 | 100 | 93 | 100 | 0 | 28 |
| Compost (7) | 100 | 100 | 100 | 100 | 0 | 43 |
| Human feces (24) | 100 | 100 | 0 | 0 | 54 | 4 |
| Bovine feces (10) | 100 | 100 | 0 | 0 | 0 | 100 |
| Ovine feces (10) | 100 | 100 | 0 | 0 | 0 | 100 |
| Equine feces (10) | 100 | 100 | 0 | 0 | 0 | 0 |
A result was considered positive when marker concentration was greater than 4.5 log10 copies per g in feces and compost and 3.5 log10 copies per 100 ml of water.