TABLE 2.
Efficiency of transduction and infection of S. aureus wild-type and hsdR mutant strains using phages 80α and φ75
| Strain | Transduction of plasmid pGC2 from strain 879R4RF (no. of transductants/106 phage particles)
|
Efficiency of plaquinga
|
||
|---|---|---|---|---|
| Phage 80α | Phage φ75 | Phage 80α from 879R4RF | Phage φ75 from 879R4RF | |
| RN4220 | 158 | 620 | 1.0 | 1.0 |
| NCTC8325-4 | 0 | 0 | 3.1 × 10−5 | 3.7 × 10−6 |
| NCTC8325-4ΔHsdR | 0 | 0 | 5.8 × 10−5 | 3.9 × 10−3 |
| NCTC8325-4ΔC-term HsdR | 0 | 0 | 1.0 × 10−4 | 6.1 × 10−3 |
| SH1000 | 0 | 0 | 4.5 × 10−5 | 4.7 × 10−5 |
| SH1000ΔHsdR | 0 | 0 | 1.6 × 10−4 | 3.6 × 10−3 |
a
The efficiency of plaquing was calculated by dividing the number of phage PFU per milliliter of phage lysate in each strain tested by the corresponding number in RN4220.