TABLE 3.
Enzyme specific activities of crude extracts of E. coli BL21Star(DE3) and MG1655(DE3)
| Enzyme | Sp act(U mg−1) for E. coli straina:
|
|
|---|---|---|
| BL21Star(DE3) | MG1655(DE3) | |
| Acetoacetyl-CoA thiolase | ||
| Control | 0.04 ± 0.01 | 0.03 ± 0.00 |
| BktB | 5.05 ± 0.69 | 1.10 ± 0.24 |
| Thl | 29.66 ± 3.87 | 6.85 ± 0.97 |
| PhaA | 5.90 ± 0.87 | 2.56 ± 0.36 |
| 3-HB-CoA dehydrogenase | ||
| Control | NDb | ND |
| Hbd | 58.78 ± 7.76 | 40.76 ± 8.08 |
| PhaB | 0.41 ± 0.18 | 0.33 ± 0.05 |
a
One unit was defined as the conversion of 1 μmol of substrate to product per min at 25°C. Data are presented as the average value and standard deviation of measurements from three independent cultures.
b
ND, not detected.