TABLE 4.
Levels and ratios of NAD+, NADH, NADP+, and NADPH cofactors in engineered MG1655(DE3) strainsa
| Construct and postinduction time | Cofactor level (nmol/mg DCW)
|
Cofactor ratio
|
||||
|---|---|---|---|---|---|---|
| NAD+ | NADH | NADP+ | NADPH | NADH/NAD+ | NADPH/NADP+ | |
| pET-H-P + pCDF-T | ||||||
| 4 h | 6.15 ± 0.75 | 0.97 ± 0.11 | 1.58 ± 0.16 | 0.50 ± 0.08 | 0.16 ± 0.00 | 0.31 ± 0.02 |
| 24 h | 3.95 ± 0.29 | 1.75 ± 0.02 | 0.96 ± 0.08 | 0.94 ± 0.09 | 0.44 ± 0.03 | 0.99 ± 0.18 |
| pET-P-P + pCDF-T | ||||||
| 4 h | 7.11 ± 0.08 | 0.59 ± 0.07 | 1.89 ± 0.18 | 0.49 ± 0.03 | 0.08 ± 0.01 | 0.26 ± 0.01 |
| 24 h | 4.94 ± 0.13 | 1.49 ± 0.21 | 1.22 ± 0.05 | 0.97 ± 0.23 | 0.30 ± 0.03 | 0.79 ± 0.16 |
| pETDuet + pCDFDuet | ||||||
| 4 h | 8.05 ± 1.09 | 0.85 ± 0.08 | 2.12 ± 0.04 | 0.59 ± 0.02 | 0.11 ± 0.00 | 0.28 ± 0.02 |
| 24 h | 4.79 ± 0.44 | 0.96 ± 0.06 | 1.12 ± 0.12 | 0.97 ± 0.09 | 0.20 ± 0.01 | 0.87 ± 0.01 |
a
Data are presented as the average and standard deviation of measurements from two independent cultures.