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. 2009 Mar 13;75(10):3358–3361. doi: 10.1128/AEM.02538-08

FIG. 1.

FIG. 1.

RT-PCR assays were carried out on RNA isolated from various L. lactis strains. (A) Expression of AbiV. (B) Control experiment performed in the absence of RT. (C) Expression of gene tnp981. (A to C) Lane 1, L. lactis JH-80 (spontaneous BIM); lane 2, L. lactis JH-32 (insertional mutant expressing abiV); lane 3, L. lactis JH-20 (abiV gene cloned into an expression vector); lane 4, L. lactis JH-54 (empty vector); lane 5, positive PCR control using genomic DNA from L. lactis MG1363; lane L, GeneRuler ladder (Fermentas). (D) Detection of increasing lengths of abiV transcripts using the same forward primer in abiV and progressively more-distant reverse primers. (D) Lane a, forward and reverse (rev) primers located in abiV; lane b, rev primer 75 bp upstream of abiV; lane c, rev primer 310 bp upstream of abiV; lane d, rev primer 727 bp upstream of abiV; lane e, rev primer 1,079 bp upstream of abiV; lane L, GeneRuler ladder (Fermentas).