FIG. 2.

abiV and its upstream region, with tnp designating tnp981. The abiV transcription start site was shown with RT-PCR and RACE. Lines a to d represent the results of the four positive reactions and line e represents the result of the negative reaction shown in Fig. 1D. Two sizes of transcripts were obtained by using RACE. Vertical lines symbolize the range of observed mutations in tnp981 that were likely caused by a recombination event with the very similar, inversely oriented transposase located 5.5 kb upstream of tnp981. Numbers refer to nucleotide numbers in GenBank AM406671. Figure not drawn to scale.