TABLE 2.

Comparative gene expression profiles of NCFM and the Δupp mutant NCK1909 grown in MRS medium

Open reading frame	Annotation	Δupp/NCFM expression ratio	Log2 ratio	P value
Gene expression in early log phase (OD600, ∼0.3)
Open reading frames expressed at higher levels in the Δupp mutant than in strain NCFM
1564	Membrane protein	2.05	1.04	0.005
1970	Hypothetical protein (49 amino acids)	2.03	1.02	0.02
Open reading frames expressed at lower levels in the Δupp mutant than in strain NCFM
1873	Acetate kinase	0.49	−1.02	0.0002
1568	Putative surface protein	0.48	−1.05	0.0006
0586	Galactose-1-phosphate uridylyltransferase	0.47	−1.09	1.06E-06
1066	Putative phosphorylase	0.47	−1.10	2.73E-05
1871	Neopullulanase	0.36	−1.49	9.41E-05
1362	Hypothetical protein	0.31	−1.67	1.41E-05
Gene expression at mid-log phase (OD600, ∼0.8)
ORFs expressed at lower levels in the Δupp mutant than in strain NCFM
1357	ABC transporter ATPase and permease protein	0.499	−1.00	0.004