TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or referencea |
|---|---|---|
| X. euvesicatoria | ||
| E3-1 | Nalr Smr | 9 |
| 91-106 | 36 | |
| X. perforans | ||
| 91-118 | Rifr | 36 |
| 91-118ΔbcnB | BcnB− Rifr Kmr | 9 |
| 91-118ΔopgH | OpgH− Rifr | 23 |
| 91-118ΔopgHΔbcnB | OpgH− BcnB− Rifr Kmr | This study |
| E. coli | ||
| DH5α | F−recA | BRL |
| C2110 | Nalr | BRL |
| λPIR | Host for pOK1; Spr oriR6K RK2 replicon UB | |
| Plasmids | ||
| pOK1 | Suicide vector; SacB | 10 |
| pBluescript-KS+ | Phagemid, pUC derivative; Ampr | Stratagene |
| pLAFR3 | Tcrrlx+ RK2 replicon | BJS |
| pRK2013 | Helper plasmid; Kmr Tra+ | 4 |
| pXV442-255 | pLAFR3; bcnB with Kmr insertion | STN |
a
BRL, Bethesda Research Laboratories, Gaithersburg, MD; Stratagene, Stratagene Inc., La Jolla, CA; BJS, B. J. Staskawicz, University of California, Berkeley; UB, U. Bonas, Martin-Luther-Universität, Halle, Germany; STN, Simone Tudor-Nelson.