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. 2009 Mar 27;75(10):3314–3322. doi: 10.1128/AEM.02632-08

FIG. 1.

FIG. 1.

Schematic representation of homologous recombination events in A. baylyi reporter strains used in this study. (A) A. baylyi strain BD413(pBAB2) harbors the pBAB2 plasmid (11) which contains two neighboring chloroplastic gene sequences (accD and rbcL), favoring recombination with homologous sequences of the transplastomic tobacco DNA flanking the aadA transgene. After homologous recombination with plant DNA, the transgene is inserted in the plasmid and confers a spectinomycin-resistant phenotype (Sp+) to the transformed cell. (B) A. baylyi strain BD413(rbcL-ΔPaadA::gfp) carries a promoterless aadA::gfp fusion downstream of rbcL, cloned in the chromosome between the lipB and the lipA genes. The functionality of the aadA::gfp fusion is restored after homologous recombination with the transgenic DNA, conferring both antibiotic-resistant and green fluorescent (gfp+) phenotypes to the recombinant cells.