TABLE 1.
Transformation experiments with A. baylyi strain BD413(rbcL-ΔPaadA::gfp) and A. baylyi strain BD413(pBAB2) exposed to plasmid pCEA and to different sources of transplastomic and wild-type plant DNA
| A. baylyi straina | Donor DNAb | Amt (material) | No. of transformants per assayc | Transformation frequencyc |
|---|---|---|---|---|
| BD413(rbcL-ΔPaadA::gfp) | Linear pCEA plasmid | 1 μg (DNA) | (1.3 ± 0.6) × 105 | (1.4 ± 0.4) × 10−5 |
| Total DNA from TP leaf tissue | 5 μg (DNA) | (1.3 ± 0.2) × 102 | (2.4 ± 0.4) × 10−8 | |
| TP ground leaves | 500 mg | (3.2 ± 1.9) × 101 | (8.4 ± 2.2) × 10−10 | |
| WT ground leaves | 500 mg | 0 | <10−10 | |
| BD413(pBAB2) | Linear pCEA plasmid | 1 μg (DNA) | (5.0 ± 0,1) × 105 | (1.7 ± 0,8) × 10−3 |
| TP total DNA from leaf tissue | 5 μg (DNA) | (6.0 ± 0.8) × 102 | (4.1 ± 2.3) × 10−6 | |
| TP ground leaves | 500 mg | (1.0 ± 0.1) × 101 | (1.5 ± 6.0) × 10−8 | |
| WT ground leaves | 500 mg | 0 | <10−10 |
a
Recipient concentrations were 3.4 × 107 CFU/ml and 7.0 × 108 CFU/ml for A. baylyi strain BD413(rbcL-ΔPaadA::gfp) and A. baylyi strain BD413(pBAB2), respectively.
b
TP, transplastomic; WT, wild-type.
c
Data are means ± standard deviations from three independent experiments.