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Exopolyphosphatase activity and PolyP accumulation in various strains of C. glutamicum. Open columns, exopolyphosphatase activity; filled columns, PolyP content expressed in millimolar Pi. Average values and standard deviations of at least three independent determinations are shown. Enzyme activity was measured spectrophotometrically using the EnzChek phosphate assay kit in 1 ml containing 50 mM PIPES, pH 6.8, 25 mM KCl, 2 mM MgCl2, and 40 mM PolyP20. *, P < 0.05; **, P < 0.005.
