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Purification of PPX2His of C. glutamicum from E. coli BL21(DE3) (pET16b-ppx2). (Lane 1) SeaBlue Plus2 prestained standard (Invitrogen) containing proteins of the indicated molecular masses. Also shown is Coomassie blue-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein from extracts before (lane 2) and 4 h after (lane 3) (both 150 μl OD600−1) induction with 0.5 mM IPTG and 12 μg PPX2His purified by Ni-nitrilotriacetic acid chromatography (lane 4).
