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Influence of PolyP chain length on the activity of PPX2His. For PolyP standards, see Materials and Methods. Enzyme activity was measured spectrophotometrically using the EnzChek phosphate assay kit in 1 ml containing 50 mM PIPES, pH 6.8, 25 mM KCl, 2 mM MgCl2, up to 15 μg PPX2His, and up to 40 mM PolyP.
