FIG. 4.

Biomass formation (grams dry weight per liter) of C. glutamicum strains WT(pVWEx1), WT(pVWEx1-ppx1) and WT(pVWEx1-ppx2) (A and B), the WT, and the Δppx1 and Δppx2 strains (C and D), after transfer to minimal medium with different phosphate concentrations. Cells cultured overnight on LB medium were used to inoculate CgXII minimal medium with 4% (wt/vol) glucose and 0.13 mM (A and C) or 0 mM (B and D) phosphate. Cultivations shown in panels A and B were performed in the presence of 25 μg/ml kanamycin and 1 mM IPTG. Biomass formation was determined after 12 to 13 h of incubation. Averages and standard deviations of at least three independent cultivations are shown. *, P < 0.05; **, P < 0.005.