TABLE 4.
Impacts of genetic modifications on ethanol production and corresponding acetoin, acetate, and glycerol levels produced during alcoholic fermentationa
| Strain | Concn (g/liter) of:
|
Ethanol content (°) in:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Acetoin
|
2,3-BD
|
Glycerol
|
Ethanol
|
|||||||
| 200-g/liter- glucose medium | 240-g/liter- glucose medium | 200-g/liter- glucose medium | 240-g/liter- glucose medium | 200-g/liter- glucose medium | 240-g/liter- glucose medium | 200-g/liter- glucose medium | 240-g/liter- glucose medium | 200-g/liter- glucose medium | 240-g/liter- glucose medium | |
| V5 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.5 ± 0.1 | 1.3 ± 0.4 | 5 ± 0.08 | 7 ± 0.6 | 97 ± 0.3 | 119 ± 1.4 | 12.2 | 15.0 |
| V5 ald6 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.7 ± 0.1 | 1.4 ± 0.3 | 6.3 ± 0.6 | 8 ± 0.3 | 97 ± 0.3 | 117 ± 1.6 | 12.2 ± 0.03 | 14.8 ± 0.2 |
| V5 ald6 GPD1 | 5.9 ± 1.5 | 7.6 ± 1.7 | 1.3 ± 0.3 | 2.4 ± 0.6 | 27 ± 0.2 | 33 ± 0.4 | 78 ± 0.5 | 99 ± 0.1 | 9.8 | 12.4 |
| V5 ald6 GPD1 BDH1 | 1.0 ± 0.07 | 0.8 ± 0.0 | 6.0 ± 0.9 | 8.9 ± 0.5 | 24 ± 0.14 | 29 ± 1.1 | 80 ± 0.6 | 98 ± 0.2 | 10.1 | 12.4 |
| V5 ald6 GPD1 BDH1221,222,223 | 1.2 ± 0.36 | 0.9 ± 0.14 | 7.0 ± 2.3 | 8.9 ± 1.6 | 26 ± 0.45 | 32 ± 0.0 | 79 ± 0.0 | 97 ± 0.7 | 9.9 | 12.2 |
a
Experiments were carried out with MS media containing 200 and 240 g/liter glucose. The means ± standard deviations of results from two independent experiments are shown.