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Primers used for real-time PCR
| Gene | Amplicon size (bp) | Forward primer | Reverse primer |
|---|---|---|---|
| purA | 169 | CCCAATTGCTGGTGGTGTAAC | TCCATACTCGCGACCAACTTC |
| purC | 165 | TTTCGCAAGCTGTCGTGTAAG | CCAAGATCACGACGGAATACG |
| purL | 162 | AAGCACAGTTGTTACGCCAG | CATACGATATTACGCGCTGCC |
| 16S rRNA | 162 | TAGGTGGCAAGCGTTATCCG | GCATTTCACCGCTACACATGG |
