FIG. 1.

Serial batch subculture of P. syringae pv. tomato DC3000 in hrp-inducing minimal medium with 0.2% fructose demonstrates a carryover effect from seed culture grown in KB medium. The first serial culture was derived by transferring 1.0 ml of seed culture into KB medium (OD₆₀₀, 2.19 ± 0.09) to 50.0 ml of freshly prepared hrp-inducing minimal medium without a washing step. The second and third serial cultures were generated by inoculating the same amount (OD₆₀₀) of the corresponding parental cultures to 50.0 ml of freshly prepared hrp-inducing minimal medium (see inset table). •, first serial culture; ▴, second serial culture; ▪, third serial culture.