FIG. 3.

The amounts of RNA transcripts for four selected genes are compared between a steady-state culture (the collection time was between −10 and −7 h before the pulse injection) in hrp-inducing minimal medium with 0.2% fructose without iron and a pulse-perturbed continuous culture (the collection time was 0 to 3 h or 3 to 6 h after the pulse injection) by using a pulse of 50 μM iron oxalate with a dilution rate of 0.070 ± 0.003 h⁻¹. The amounts of mRNA of each gene were normalized to gyrA mRNA levels. Log₁₀ difference was calculated as log₁₀ (normalized amounts of RNA of test gene from pulse/normalized amounts of RNA of test gene from steady state). Each column bar represents an independent experiment set (three biological replicates). Error bars represent the standard deviations for three technical replicates for each independent experiment set. gyrA, a general housekeeping gene; hrpL, the major regulatory gene for T3SS; hopAA1-1, a T3SS effector gene; PSPTO2134, a pyoverdine biosynthesis gene.