TABLE 1.

Sequence similarities of the presumptive DNA polymerase activity-conferring gene products encoded by pCS1 to pCS8 and by fCS1 to sequences of gene products from other organisms^a

Plasmid or fosmid	Insert size (kb)	No. of. amino acids of polymerase gene product	Detected domain(s)b	Closest similar protein, no. of amino acids (accession no. of similar protein)	Organism	Region of similar amino acids (% identity)	E value
pCS1	3.5	557	53 exo, 35 exo	Putative DNA polymerase I, 941 (ZP_01718371)	Algoriphagus sp. strain PR1	8-521 (52)	1e-155
pCS2	15.0	944	53 exo, 35 exo, DNA_polA	DNA polymerase I, 955 (ZP_01689558)	Microscilla marina ATCC 23134	1-955 (58)	0
pCS3	6.3	803	53 exo, DNA_polA	DNA polymerase I, 833 (YP_144320)	Thermus thermophilus HB8	7-827 (35)	5e-124
pCS4	4.8	282	53 exo	Exodeoxyribonuclease, 276 (AAX12058)	Bacteriophage T5	2-257 (43)	8e-52
pCS5	6.4	927	53 exo, 35 exo, DNA_polA	DNA polymerase I, 923 (YP_001845275)	Acinetobacter baumannii ACICU	1-923 (65)	0
pCS6	4.6	942	53 exo, 35 exo, DNA_polA	DNA polymerase I, 937 (ZP_01884419)	Pedobacter sp. strain BAL39	2-937 (57)	0
pCS7	6.2	646	53 exo, 35 exo, DNA_polA	DNA polymerase I, 920 (AAG43148)	Rhodococcus erythropolis	1-647 (82)	0
pCS8	9.0	927	53 exo, 35 exo, DNA_polA	DNA polymerase I, 923 (YP_001845275)	Acinetobacter baumannii ACICU	1-923 (66)	0
fCS1	32.0	962	53 exo, 35 exo, DNA_polA	Putative DNA polymerase I, 941 (ZP_01718371)	Algoriphagus sp. strain PR1	8-941 (57)	0

^aThe determinations of the values for the percentages of identity, the regions of similar amino acids, and the E values were performed by using BLAST programs (1). The domains were detected by searches of the CDD database (15).

^bThe E values for the domains are given in Fig. 1.