FIG. 3.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel of OM proteins obtained from cultures of V. anguillarum RV22 (wild type [WT]), ΔfvtA (MB84), Δorf13 (MB70), and ΔfvtA Δorf13 (MB90) mutants, and the MB84 mutant complemented with plasmid pMB54 under iron-sufficient (Fe+) and iron-deficient (Fe−) conditions. The arrows indicate the locations of the bands corresponding to the iron-regulated putative FvtA protein. The numbers on the left indicate the molecular masses of the protein markers.