FIG. 5.

Detection of fvtA transcripts using RT-PCR. Fourteen V. anguillarum strains belonging to serotypes O1, O2, and O3 were analyzed (strain designations are indicated at top). The arrow on the left indicates the expected size of the fvtA RT-PCR product. The same RT-PCR carried out with Vibrio ordalii 13A5 was negative. Negative controls using DNase-treated RNA as the PCR template to rule out the presence of contaminating DNA were negative in all cases (an example is the control for RV22 [⊘]). The ladder is composed of 10 fragments whose sizes range from 100 to 1,000 bp in 100-bp increments.