TABLE 1.
Bacterial strains and plasmids
| Plasmid or strain | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Plasmids | ||
| pGEMT-Easy | PCR cloning vector, Ampr | Promega |
| pWKS30 | Low-copy-number cloning vector, Ampr | 44 |
| pNidKan | pir-dependent suicide plasmid, pCVD442 derivative, sacB, Kanr | 27 |
| pHRP309 | Low-copy-number vector, Gmr | 30 |
| MBcos167 | Cosmid containing orf13 | 3 |
| MBcos69 | Cosmid containing vabD | 3 |
| pMB54 | fvtA gene and promoter cloned in pHRP309, Gmr | This study |
| V. anguillarum strains | ||
| RV22 | Serotype O2 strain isolated from diseased turbot | 20 |
| MB11 | RV22 ΔvabB | 4 |
| MB84 | RV22 ΔfvtA | This study |
| MB70 | RV22 Δorf13 | This study |
| MB90 | RV22 ΔfvtA Δorf13 | This study |
| MB102 | RV22 ΔvabB ΔfvtA | This study |
| MB104 | RV22 ΔvabB Δorf13 | This study |
| MB107 | RV22 ΔvabB ΔfvtA Δorf13 | This study |
| E. coli S17-1-λpir | Tpr Smr, recA thi pro hsdR-M+RP4::2-Tc::Mu::Km Tn7 λpir | 19 |