TABLE 2.
Substrate | % Activity at inhibitor concn of:
|
|
---|---|---|
1.0 mM | 0.25 mMb | |
EDTA (disodium salt) | 81 | ND |
Ascorbic acid | 76 | ND |
Dithiothreitol | 76 | ND |
β-Mercaptoethanol | 75 | ND |
Dithionite | 25 | 8 |
Diethyldithiocarbamate | 50 | 61 |
Dipyridyl | 46 | 59 |
Dodecanol | 54 | 55 |
Assays were run in 100 mM MES buffer (pH 6.5) with 200 μM NADPH, 250 μM decanal, and 125 μg of FALDR fusion protein. All compounds were dissolved in buffer, except dipyridyl, which was dissolved in dimethyl sulfoxide. Inclusion of dimethyl sulfoxide did not result in decreased activity at the same concentration. The percentage of activity reported here is the result from a single sample analysis with a specific set of data. The measurements were repeated several times to confirm reproducibility. Activity is reported as the percentage of activity remaining in the presence of each compound based on the activity without any inhibitor present.
ND, not determined.