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. 2009 Mar 6;75(9):2951–2957. doi: 10.1128/AEM.02613-08

FIG. 2.

FIG. 2.

Southern blots for vmp1 detection in the StolP genome. (A) Hybridization at high stringency with an SR1H10 digoxigenin-labeled PCR-amplified probe. Lanes: 3 and 5, healthy periwinkle total DNA; 2 and 4, StolP PO-infected periwinkle DNA; 2 and 3, undigested; 4 and 5, digested with HindIII; 1, the native pGEMT-Easy plasmid containing the SR1H10 SSH product. (B) Hybridization at low stringency with an SR1H10 digoxigenin-labeled PCR-amplified probe hybridized to HindIII-digested DNA from periwinkle infected by different StolP isolates (indicated above the lanes).