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(A) Detection of C. sakazakii 274 on a glass slide using the SakPNA971 probe, a preenriched culture (10% PIF), and an initial concentration of 1 to 3 CFU/100 ml reconstituted PIF. (B) Visualization of the same microscopic field with the green channel (negative control), showing autofluorescence of infant formula proteins and the absence of fluorescent cells.
