TABLE 4.
Real-time PCR results for three different group-specific assays
| Phytoplasma strain or bacterial source (isolate designation) | 16Sr group | DNA sourcea | Avg CT valueb
|
||
|---|---|---|---|---|---|
| Group II | Group IV | Group XII | |||
| Negative control containing water | —c | NDd | ND | ND | |
| B. gladioli | |||||
| Pure culture | — | ND | ND | ND | |
| Inoculated Allium foliage | — | ND | ND | ND | |
| Inoculated Allium bulb | — | ND | ND | ND | |
| Rehmannia glutinosa (RG) | I-B | Plant, J. Přibylová | ND | 35.7 | — |
| Black currant reversion disorder (BCRD) | I-C | Plant, J. Přibylová | ND | — | — |
| Strawberry green petal (SGP) | I-C | Plant, J. Přibylová | ND | 38.7 | ND |
| Fava bean phyllody (FBP) | II-C | Plant, P. Jones | 19.2* | 38.0 | — |
| Crotalaria saltiana phyllody (FBPSA) | II-C | Plant, R. Mumford | 19.5* | ND | — |
| Soybean phyllody (SOYP) | II-C | Plant, P. Jones | 20.9* | ND | — |
| Sweet potato little leaf (SPLL) | II-D | Plant, P. Jones | 26.7* | ND | ND |
| Sweet potato little leaf (SPLL) | II-D | Plant, P. Jones | 19.3* | — | — |
| Vinca coconut phyllody (VCP) | II-?e | Plant, P. Jones | 19.3* | ND | — |
| Peach western X (CX) | III-A | DNA, A. Bertaccini | — | ND | — |
| Poinsettia branching factor (JR1) | III-H | Commercial plant, United Kingdom | ND | — | ND |
| Coconut lethal yellowing (Adonidia merrillii) (LYAM) | IV-A | DNA, N. Harrison | — | 20.0* | — |
| Coconut lethal yellowing (Cocus nucifera) (LYCN) | IV-A | DNA, N. Harrison | — | 19.9* | — |
| Coconut lethal yellowing (Hyophorbe verschaffeltii) (LYHV) | IV-A | DNA, N. Harrison | ND | 21.7* | ND |
| Coconut lethal yellowing (Phoenix rupicola) (LYPR) | IV-A | DNA, N. Harrison | — | 18.9* | — |
| Tanzanian coconut lethal decline (Kifumangao) (TLD1) | IV-B | DNA, P. Jones | — | 26.6* | — |
| Tanzanian coconut lethal decline (Chambezi) (TLD2) | IV-B | DNA, P. Jones | — | 23.1* | — |
| Ghanaian Cape St. Paul wilt (Daboase) (CSPWD1) | IV-C | DNA, J. Nipah | ND | 20.6* | — |
| Ghanaian Cape St. Paul wilt (Fasen) (CSPWD2) | IV-C | DNA, J. Nipah | — | 20.8* | — |
| Ghanaian Cape St. Paul wilt (Dixcove) (DIXCOVE) | IV-C | DNA, P. Jones | — | 18.2* | — |
| Nigerian coconut lethal decline (LDN) | IV-C | DNA, P. Jones | — | 18.2* | — |
| Rubus stunt (RuS) | V | DNA, A. Bertaccini | — | 37.0 | — |
| Brinjal little leaf (BLL) | VI-A | Plant, P. Jones | ND | 36.3 | — |
| Ash yellows (ASHY-1) | VII-A | DNA, A. Bertaccini | — | 36.6 | — |
| Pigeonpea witches’ broom (PPWB) | IX | DNA, N. Harrison | ND | — | — |
| Flower stunting (BVK) | XI | DNA, A. Bertaccini | — | ND | — |
| Napier grass stunt (NGS) | XI | Plant, P. Jones | ND | — | ND |
| Stolbur of pepper (STOL) | XII-A | DNA, A. Bertaccini | — | ND | 21.3* |
| Cordyline phytoplasma (CPF) | XII | DNA, R. Mumford | — | — | 23.8* |
a
Samples were obtained as DNA preparations (DNA) or plant material (Plant) from the people named, and their affiliations are given in Acknowledgments.
b
*, the assay should detect these isolates.
c
—, not tested.
d
ND, not detected, i.e., above the maximum CT value of 40 cycles.
e
?, subgroup not determined.