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Frequency distributions of lag time (tC1) for single spores of nonproteolytic C. botulinum Eklund 17B. Spores were unheated and incubated in PYGS medium at 22, 15, 10, and 8°C or were heated at 80°C for 20 s (∼10-fold reduction) and incubated in PYGS medium at 22°C (HT-22°C).
