TABLE 2.
Genes defined as essential in vivo by transposon site hybridization that contain nsSNPs across all BCG strainsc
| Gene | Rv coding sequence | BCG coding sequence | SNP | Function |
|---|---|---|---|---|
| nrp | Rv101 | BCG0134 | L1365M | Nonribosomal peptide synthase |
| senX3 | Rv0490 | BCG0531 | F109S | Two-component sensor |
| kdpD | Rv1028c | BCG1085c | P83S; N776Da | Two-component sensor |
| murI | Rv1338 | BCG1400 | R154L | Glutamate racemase |
| lysX | Rv1640c | BCG1679c | D769Ea | Lysyl-tRNA synthase |
| pks12 | Rv2048c | BCG2067c | S2964R | Mannosyl-β-1-phosphoisoprenoid synthase |
| fadE22 | Rv3061c | BCG3086c | K488Ea,b; S497Cb | Acyl coenzyme A dehydrogenase |
| Rv3335c | Rv3335c | BCG3406c | A86Va | Integral membrane protein |
| Rv3616c | Rv3616c | BCG3680c | A4Va | ESX-1 secreted antigen |
a
Conservative substitution.
b
Position with reference to H37Rv protein sequence as M. bovis 2122/97 allele is frameshifted.
c
Transposon site hybridization was performed as described previously (50).