FIG. 2.

Effect of adjuvants on intracellular cAMP. The level of intracellular cAMP increases upon treatment with CT, LT, or FSK. Stimulated B cells were treated with the compounds indicated for 24 h in the presence of 3-isobutyl-1-methylxanthine and lysed with HCl. The amount of cAMP was evaluated by an enzyme immunoassay and was expressed in fmol/10⁶ B cells. The asterisks indicate statistically significant differences (P < 0.05) between CT-, LT-, or FSK-treated B cells and untreated B cells (NT). The P values indicate the statistically significant differences between FSK- and CT-treated cells and between FSK- and LT-treated cells. Representative data from three independent experiments are shown.