FIG. 2.

Enhanced lung clearance of P. murina in Hck^−/− Fgr^−/− Lyn^−/− and Lyn^−/− mice. C57BL/6 (WT) and Hck^−/− Fgr^−/− Lyn^−/− (Src TKO) mice were inoculated with 2 × 10⁵ P. murina cysts via the intratracheal route. (A and B) At 3 days (A) and 7 days (B) postinoculation, lungs were collected, and the P. murina burden was determined by using real-time PCR to determine the P. murina rRNA copy number. The data are cumulative data from three independent studies with five mice per group. The bars and error bars indicate the mean P. murina rRNA copy numbers and the standard errors of the means. ** and ***, P < 0.01 and P < 0.001, respectively (unpaired two-tailed Student's t test). (C) C57BL/6 and Lyn^−/− mice were inoculated with 2 × 10⁵ P. murina cysts via the intratracheal route, and 3 days postinoculation lungs were collected and the P. murina burden was determined by using real-time PCR to determine the P. murina rRNA copy number. The data are cumulative data from three independent studies with five mice per group. The bars and error bars indicate the means and standard errors of the means. **, P < 0.01 (unpaired two-tailed Student's t test).