FIG. 5.

Hck^−/− Fgr^−/− Lyn^−/− alveolar macrophages are more efficient at killing P. murina. Alveolar macrophages were isolated from 6- to 8-week-old, male C57BL/6 (WT) or Hck^−/− Fgr^−/− Lyn^−/− (Src TKO) mice and cocultured overnight with P. murina at a ratio of macrophages to P. murina cysts of 100:1. The controls included P. murina cultured in the absence of macrophages. After this, RNA was isolated from the contents of each well, and quantitative real-time PCR to determine the P. murina rRNA copy number was performed. The data are the cumulative results from three independent studies. The bars and error bars indicate the means and standard errors of the means. **, P <0.01 (unpaired two-tailed Student's t test).