TABLE 1.
F. tularensis LVS cloud aerosol infection parameters
| CFU in nebulizera | No. of experimentsb | Day 0 uptakec | Survival (%) | MTDd (n) |
|---|---|---|---|---|
| 109 | 3 | 5.7 × 102 ± 9.7 × 101 | 1/15 (7) | 9 ± 3 (14) |
| 108 | 4 | 1.5 × 102 ± 3.5 × 101 | 4/28 (14) | 13 ± 2 (24) |
| 107 | 6 | 1.0 × 101 ± 2 × 100 | 30/35 (86) | 12 ± 2 (5) |
Total number of viable bacteria placed in the nebulizer in a 10-ml volume.
Total number of separate aerosol infection experiments included in the calculations shown here; only experiments using male C57BL/6J mice are included in this table.
Average ± SEM of CFU of bacteria delivered to the lungs following aerosol exposure, based on plate count of either dilutions or the entire homogenate of both lungs from individual mice, as needed. For the 107 dose, 5 of 26 mice(19%) sacrificed on day zero had no detectable colonies in the total lung homogenate; for these samples, no value was included in the uptake calculation shown for this dose.
MTD, mean time to death, with the day of infection designated as day zero; data are the average day of death ± SEM. Only mice that died were included in the calculation. n is the number of mice included in the mean time to death calculation(see survival column).