FIG. 4.

Antibody depletion experiment. A pool of sera from mice (n = 30) immunized with 15% TrL3 OMVs was incubated in microwells coated with whole-cell preparations from either the capsule-deficient L3,7 LOS-expressing H44/76 strain or the capsule- and LOS-deficient H44/76 mutant or purified TrL3 LOS. After 4 h of incubation, sera were tested in an SBA using the L3,7 LOS-expressing strain M97-250687 (P1.19,15). The percentage of inhibition of the bactericidal activity is shown for each antigen (representative data from one of three experiments).