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. 2009 Feb 17;77(5):1746–1756. doi: 10.1128/IAI.01530-08

FIG. 1.

FIG. 1.

A. phagocytophilum NCH-1 and NCH-1A express both full-length and N-terminally truncated Msp2 isoforms. (A) NCH-1 and NCH-1A whole-cell lysates were screened via Western blot analysis using anti-Msp2 MAb 20B4, which recognizes full-length and truncated Msp2, or MAb 8E8, which recognizes only the full-length isoform. (B and C) Uninduced whole-cell lysates of E. coli (lane ctrl) or lysates of cells induced to express recombinant full-length Msp2 or portions of recombinant full-length Msp2, including the N-terminal conserved region (lane N), the HVR (lane HVR), the C-terminal conserved region (lane C), the N terminus through the HVR (lane N-HVR), and the HVR through the C terminus (lane HVR-C), were screened via Western blot analysis using an MAb targeting the Strep-Tag II epitope (B) or MAb 8E8 (C). Ab, antibody.