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. 2009 Feb 17;77(5):1746–1756. doi: 10.1128/IAI.01530-08

FIG. 2.

FIG. 2.

Two-dimensional SDS-PAGE and Western blot analyses of A. phagocytophilum NCH-1 Msp2(P44) proteins. Hydrophobic pellet fractions enriched for A. phagocytophilum NCH-1 outer membrane proteins were isoelectric focused (IEF) in IPG strips (pH 5.0 to 8.0), which was followed by resolution in the second dimension in SDS-polyacrylamide (4 to 20%) gels. (A) Silver-stained gel. Msp2 proteins that were excised for LC-MS/MS identification or detected by anti-Msp2(P44) antibodies are indicated by a box. (B) Enlarged view of the region of the silver-stained gel in panel A indicated by the box. Numbered spots were excised and identified by LC-MS/MS. (C) MAb 20B4 recognized spots in the predicted size range for full-length Msp2(P44) proteins, as well as multiple 25- to 27-kDa spots.